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needle electrodes 29g  (ADInstruments)


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    ADInstruments needle electrodes 29g
    Cardiac cell sheet implantation . (a) An endothelial cell network (evaluated from the expression of CD31, an endothelial cell marker) was clearly detected following the co-culture of green fluorescent protein (GFP)-expressing human umbilical vein endothelial cells (HUVECs) with adult normal human dermal fibroblasts (NHDFs) at a ratio of 10:1. cTnT: cardiac troponin T. (b) An endothelial cell network was not detected after the co-culture of cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) and GFP-positive HUVECs. CM: cardiomyocytes. (c) An endothelial cell network was created when cardiomyocytes were co-cultured with GFP-positive HUVECs and NHDFs at a ratio of 5:1:5. (d) Cardiomyocytes derived from hiPSCs were co-cultured with GFP-positive HUVECs in a temperature-responsive dish and harvested as a monolayer sheet. The harvested sheet shrank to approximately one-third of the dish diameter. (e) Three harvested cardiac cell sheets were layered, and this triple-layered cell sheet (broken circle) was placed onto a vascular bed created from porcine small intestine (day 0). The fabricated tissue construct was then subjected to perfusion culture for 6 days. (f) Needle <t>electrodes</t> were used to record surface action potentials from the implanted cardiac cell sheet (broken circle) after 6 days of perfusion culture. (g) Representative trace showing surface action potentials recorded from the implanted cardiac cell sheet after 6 days of perfusion culture. The implanted cardiac cell sheet was observed to beat spontaneously with a regular rhythm.
    Needle Electrodes 29g, supplied by ADInstruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/needle electrodes 29g/product/ADInstruments
    Average 90 stars, based on 1 article reviews
    needle electrodes 29g - by Bioz Stars, 2026-04
    90/100 stars

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    1) Product Images from "Generation of a large-scale vascular bed for the in vitro creation of three-dimensional cardiac tissue"

    Article Title: Generation of a large-scale vascular bed for the in vitro creation of three-dimensional cardiac tissue

    Journal: Regenerative Therapy

    doi: 10.1016/j.reth.2019.10.001

    Cardiac cell sheet implantation . (a) An endothelial cell network (evaluated from the expression of CD31, an endothelial cell marker) was clearly detected following the co-culture of green fluorescent protein (GFP)-expressing human umbilical vein endothelial cells (HUVECs) with adult normal human dermal fibroblasts (NHDFs) at a ratio of 10:1. cTnT: cardiac troponin T. (b) An endothelial cell network was not detected after the co-culture of cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) and GFP-positive HUVECs. CM: cardiomyocytes. (c) An endothelial cell network was created when cardiomyocytes were co-cultured with GFP-positive HUVECs and NHDFs at a ratio of 5:1:5. (d) Cardiomyocytes derived from hiPSCs were co-cultured with GFP-positive HUVECs in a temperature-responsive dish and harvested as a monolayer sheet. The harvested sheet shrank to approximately one-third of the dish diameter. (e) Three harvested cardiac cell sheets were layered, and this triple-layered cell sheet (broken circle) was placed onto a vascular bed created from porcine small intestine (day 0). The fabricated tissue construct was then subjected to perfusion culture for 6 days. (f) Needle electrodes were used to record surface action potentials from the implanted cardiac cell sheet (broken circle) after 6 days of perfusion culture. (g) Representative trace showing surface action potentials recorded from the implanted cardiac cell sheet after 6 days of perfusion culture. The implanted cardiac cell sheet was observed to beat spontaneously with a regular rhythm.
    Figure Legend Snippet: Cardiac cell sheet implantation . (a) An endothelial cell network (evaluated from the expression of CD31, an endothelial cell marker) was clearly detected following the co-culture of green fluorescent protein (GFP)-expressing human umbilical vein endothelial cells (HUVECs) with adult normal human dermal fibroblasts (NHDFs) at a ratio of 10:1. cTnT: cardiac troponin T. (b) An endothelial cell network was not detected after the co-culture of cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) and GFP-positive HUVECs. CM: cardiomyocytes. (c) An endothelial cell network was created when cardiomyocytes were co-cultured with GFP-positive HUVECs and NHDFs at a ratio of 5:1:5. (d) Cardiomyocytes derived from hiPSCs were co-cultured with GFP-positive HUVECs in a temperature-responsive dish and harvested as a monolayer sheet. The harvested sheet shrank to approximately one-third of the dish diameter. (e) Three harvested cardiac cell sheets were layered, and this triple-layered cell sheet (broken circle) was placed onto a vascular bed created from porcine small intestine (day 0). The fabricated tissue construct was then subjected to perfusion culture for 6 days. (f) Needle electrodes were used to record surface action potentials from the implanted cardiac cell sheet (broken circle) after 6 days of perfusion culture. (g) Representative trace showing surface action potentials recorded from the implanted cardiac cell sheet after 6 days of perfusion culture. The implanted cardiac cell sheet was observed to beat spontaneously with a regular rhythm.

    Techniques Used: Expressing, Marker, Co-Culture Assay, Derivative Assay, Cell Culture, Construct



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    needle electrodes 29g  (ADInstruments)
    90
    ADInstruments needle electrodes 29g
    Cardiac cell sheet implantation . (a) An endothelial cell network (evaluated from the expression of CD31, an endothelial cell marker) was clearly detected following the co-culture of green fluorescent protein (GFP)-expressing human umbilical vein endothelial cells (HUVECs) with adult normal human dermal fibroblasts (NHDFs) at a ratio of 10:1. cTnT: cardiac troponin T. (b) An endothelial cell network was not detected after the co-culture of cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) and GFP-positive HUVECs. CM: cardiomyocytes. (c) An endothelial cell network was created when cardiomyocytes were co-cultured with GFP-positive HUVECs and NHDFs at a ratio of 5:1:5. (d) Cardiomyocytes derived from hiPSCs were co-cultured with GFP-positive HUVECs in a temperature-responsive dish and harvested as a monolayer sheet. The harvested sheet shrank to approximately one-third of the dish diameter. (e) Three harvested cardiac cell sheets were layered, and this triple-layered cell sheet (broken circle) was placed onto a vascular bed created from porcine small intestine (day 0). The fabricated tissue construct was then subjected to perfusion culture for 6 days. (f) Needle <t>electrodes</t> were used to record surface action potentials from the implanted cardiac cell sheet (broken circle) after 6 days of perfusion culture. (g) Representative trace showing surface action potentials recorded from the implanted cardiac cell sheet after 6 days of perfusion culture. The implanted cardiac cell sheet was observed to beat spontaneously with a regular rhythm.
    Needle Electrodes 29g, supplied by ADInstruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/needle electrodes 29g/product/ADInstruments
    Average 90 stars, based on 1 article reviews
    needle electrodes 29g - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    29g needle electrodes  (ADInstruments)
    90
    ADInstruments 29g needle electrodes
    Cardiac cell sheet implantation . (a) An endothelial cell network (evaluated from the expression of CD31, an endothelial cell marker) was clearly detected following the co-culture of green fluorescent protein (GFP)-expressing human umbilical vein endothelial cells (HUVECs) with adult normal human dermal fibroblasts (NHDFs) at a ratio of 10:1. cTnT: cardiac troponin T. (b) An endothelial cell network was not detected after the co-culture of cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) and GFP-positive HUVECs. CM: cardiomyocytes. (c) An endothelial cell network was created when cardiomyocytes were co-cultured with GFP-positive HUVECs and NHDFs at a ratio of 5:1:5. (d) Cardiomyocytes derived from hiPSCs were co-cultured with GFP-positive HUVECs in a temperature-responsive dish and harvested as a monolayer sheet. The harvested sheet shrank to approximately one-third of the dish diameter. (e) Three harvested cardiac cell sheets were layered, and this triple-layered cell sheet (broken circle) was placed onto a vascular bed created from porcine small intestine (day 0). The fabricated tissue construct was then subjected to perfusion culture for 6 days. (f) Needle <t>electrodes</t> were used to record surface action potentials from the implanted cardiac cell sheet (broken circle) after 6 days of perfusion culture. (g) Representative trace showing surface action potentials recorded from the implanted cardiac cell sheet after 6 days of perfusion culture. The implanted cardiac cell sheet was observed to beat spontaneously with a regular rhythm.
    29g Needle Electrodes, supplied by ADInstruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/29g needle electrodes/product/ADInstruments
    Average 90 stars, based on 1 article reviews
    29g needle electrodes - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

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    Cardiac cell sheet implantation . (a) An endothelial cell network (evaluated from the expression of CD31, an endothelial cell marker) was clearly detected following the co-culture of green fluorescent protein (GFP)-expressing human umbilical vein endothelial cells (HUVECs) with adult normal human dermal fibroblasts (NHDFs) at a ratio of 10:1. cTnT: cardiac troponin T. (b) An endothelial cell network was not detected after the co-culture of cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) and GFP-positive HUVECs. CM: cardiomyocytes. (c) An endothelial cell network was created when cardiomyocytes were co-cultured with GFP-positive HUVECs and NHDFs at a ratio of 5:1:5. (d) Cardiomyocytes derived from hiPSCs were co-cultured with GFP-positive HUVECs in a temperature-responsive dish and harvested as a monolayer sheet. The harvested sheet shrank to approximately one-third of the dish diameter. (e) Three harvested cardiac cell sheets were layered, and this triple-layered cell sheet (broken circle) was placed onto a vascular bed created from porcine small intestine (day 0). The fabricated tissue construct was then subjected to perfusion culture for 6 days. (f) Needle electrodes were used to record surface action potentials from the implanted cardiac cell sheet (broken circle) after 6 days of perfusion culture. (g) Representative trace showing surface action potentials recorded from the implanted cardiac cell sheet after 6 days of perfusion culture. The implanted cardiac cell sheet was observed to beat spontaneously with a regular rhythm.

    Journal: Regenerative Therapy

    Article Title: Generation of a large-scale vascular bed for the in vitro creation of three-dimensional cardiac tissue

    doi: 10.1016/j.reth.2019.10.001

    Figure Lengend Snippet: Cardiac cell sheet implantation . (a) An endothelial cell network (evaluated from the expression of CD31, an endothelial cell marker) was clearly detected following the co-culture of green fluorescent protein (GFP)-expressing human umbilical vein endothelial cells (HUVECs) with adult normal human dermal fibroblasts (NHDFs) at a ratio of 10:1. cTnT: cardiac troponin T. (b) An endothelial cell network was not detected after the co-culture of cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) and GFP-positive HUVECs. CM: cardiomyocytes. (c) An endothelial cell network was created when cardiomyocytes were co-cultured with GFP-positive HUVECs and NHDFs at a ratio of 5:1:5. (d) Cardiomyocytes derived from hiPSCs were co-cultured with GFP-positive HUVECs in a temperature-responsive dish and harvested as a monolayer sheet. The harvested sheet shrank to approximately one-third of the dish diameter. (e) Three harvested cardiac cell sheets were layered, and this triple-layered cell sheet (broken circle) was placed onto a vascular bed created from porcine small intestine (day 0). The fabricated tissue construct was then subjected to perfusion culture for 6 days. (f) Needle electrodes were used to record surface action potentials from the implanted cardiac cell sheet (broken circle) after 6 days of perfusion culture. (g) Representative trace showing surface action potentials recorded from the implanted cardiac cell sheet after 6 days of perfusion culture. The implanted cardiac cell sheet was observed to beat spontaneously with a regular rhythm.

    Article Snippet: The bioreactor was opened, and two needle electrodes (29G in diameter; ADInstruments, New South Wales, Australia) were placed over the cardiac tissue.

    Techniques: Expressing, Marker, Co-Culture Assay, Derivative Assay, Cell Culture, Construct